Molecular Analysis of Biofield Treated Eggplant and Watermelon Crops

The aim of the present study was to analyze the overall growth of these crops after biofield energy treatment. The plots were selected for the study and divided into two parts, control and treated. The control plots were left untreated, while the treated plots were exposed to Mr. Trivedi's biofield energy treatment. The eggplants were reported to have uniform colored, glossy, and greener leaves, which are bigger in size. The canopy of the eggplant was larger with early fruiting, while the fruits have uniform shape and texture compared with the control. However, the watermelon plants after the biofield treatment showed higher survival rate, with larger canopy, bright and dark green leaves compared with the untreated plants. The percentage of true polymorphism observed between control and treated samples of eggplant and watermelon seed samples were an average value of 18% and 17%, respectively. The study used DNA fingerprinting to analyze the genetic variability in both plants after biofield energy treatment. The RAPD method was used to study the genetic relationship before and after treatment. The results showed that Mr. Trivedi's biofield energy treatment has the ability to alter the plant growth rate and can be utilized in a better way as compared with the existing agricultural crop improvement techniques to improve the overall crop yield. Eggplant (Solanum melongena) and watermelon (Citrullus lanatus) were selected for the present study. Both the plants were selected from inbreed variety for all the experimental parameters. The biofield treated plot size for eggplant was 64 × 8 feet, while the control plot size was 47 × 12 feet. The treated plot size for watermelon was 64 × 16 feet, while the control plot size was 35 × 25 feet. Both the plots had the same number of plants, and were compared with respect to respective control. The control plots were left untreated, while the treated plots of eggplant and watermelon were subjected to Mr. Trivedi's biofield energy treatment. The seeds from each crop were cultivated for analysis. However, the control plants were given standard cultivation parameters such as proper irrigation, fertilizers, pesticides, and fungicides; while the treated plots were given only irrigation, without any supportive measure. DNA fingerprinting of both the plants was performed using RAPD techniques using Ultrapure Genomic DNA Prep Kit; Cat KT 83 (Bangalore Genei, India) to study the genetic relationship before and after treatment. The treated plots were subjected to Mr. Trivedi's biofield energy treatment. Mr. Trivedi provided the unique biofield treatment through his energy transmission process to the selected treated plots of both the crops. The plant samples of treated plots were assessed for the growth attributes with respect to control. Variability in different growth contributing parameters and genetic relatedness using RAPD of control and treated crops were compared. The seeds of eggplant and watermelon were cultivated under similar conditions. The vegetative growth of the crops with respect to plant canopy, the shape of leaves, flowering conditions, infection rate, etc. were analyzed and compared with respect to the plants of control plots. DNA fingerprinting isolation of plant genomic DNA using CTAB method was performed to study the genetic variability in both plants after biofield energy treatment. The leaves disc of both plants were harvested after germination, as it reached the appropriate stage. The genomic DNA from both plant leaves was isolated according to the standard cetyl-trimethyl-ammonium bromide (CTAB) method. Approximately 200 mg of plant tissues were grinded to a fine paste in approximately 500 μL of CTAB buffer. The mixture (CTAB/plant extract) was transferred to a microcentrifuge tube, and incubated for about 15 minutes.