INTERNATIONAL RICE RESEARCH INSTITUTE (IRRI)
Autoclaved rice stem nodes with prune juice provide a favorable medium for the isolation of Cercospora oryzae, the fungus causing Cercospora leaf spot or narrow brown leaf spot, and for the mass production of spores spraying of spores in late afternoon resulted in good infection on plants in the field.
ESTRADA, B. A.; OU, S. H. · 1970

Abstract
As shown by artificial inoculation, rice at different stages of growth is susceptible to C. oryzae. An incubation period of 20 days is necessary before the first few lesions appear and 30 days before the maximum number of lesions is reached. The slow growth of the fungus causes the late appearance of the disease, generally observed in the field. A method of artificial inoculation for screening varieties for resistance and a disease scale of 0 to 9 units, based upon the number of lesions, are suggested.
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