Mitochondrial transcripts and associated heteroplasmies of Ancistrus spp. (Siluriformes: Loricariidae)

(Siluriformes: Loricariidae) were identified through Illumina HiSeq2500 sequencing. Raw reads were assembled by Trinity, and mitochondrial transcripts were identified by BLASTN against two other Loricariidae mitogenomes. The data-set complements a previous paper on the assembly of mitochondrial genomes of Ancistrus spp. The nucleotide sequences of each transcript used to assemble the mitogenomes of three Ancistrus spp. fish are available in fasta format. Table 1 shows the number and length of each mitochondrial transcript, as well as the maximum number of supporting reads per individual nucleotide and the total sum of supporting reads for each transcript. The position of heteroplasmic sites is shown in Table 2, along with the read counts of each nucleotide, the gene category, and the codon position of each heteroplasmy. Protein-coding genes were analyzed according to Temperley et al. [9], and features in protein-coding genes were annotated using the web-based services MitoFish and MITOS [1,3]. The mitogenomes were annotated using MitoFish and MITOS, and features in protein-coding genes were analyzed according to Temperley et al. [9]. Heteroplasmic sites were detected using IGV, setting the software to show positions in which the frequency of the second most frequent base was equal to or higher than 10% and the total reads number were higher than 100. The data-set was generated with the financial support of the Partnerships for Enhanced Engagement in Science (PEER) initiative from the United States Agency for International Development [Grant no. PGA-2000003446] to TP. The data is available in Genbank under accession numbers KP960567, KP960568, and KP960569. The sequences of long mitochondrial transcripts from Ancistrus spp. are the first of their kind and will aid primer design for other Loricariidae species in phylogenetic studies. The data will also allow for the comparison of heteroplasmies position and frequency to other species. The start/stop codons usage, UTR, CDS, and poliA-tail length for protein-coding genes are described in the data-set. The Illumina HiSeq2500 sequencing was used to acquire the data, and the raw reads were assembled by Trinity. The experimental design, materials, and methods are described in a previous paper [6]. The selected transcripts were edited according to the information of strand orientation given by the BLASTN result, and aligned by SeaView using the built-in CLUSTAL alignment algorithm and the reference mitogenome. A CONTIG sequence was generated using the sequence information of just the transcripts of each individual fish. The sequence of the CONTIG was then manually checked for inconsistencies and gaps. The data-set is available in fasta format, and the tables presenting the location of each transcript in the mitogenomes, the frequency, location, and codon position of the detected heteroplasmic sites, and the start/stop codons usage, UTR, CDS, and poliA-tail length for each protein-coding gene are available in Tables 1, 2, and 3, respectively. The data is also available in Genbank under accession numbers KP960567, KP960568, and KP960569.